| business name | type | location |
| Abingdon Vineyard & Winery | farm, winery | Abingdon |
| Anderson Grass Fed Beef | farm | Bland |
| Atlantic Breeze Alpacas | farm | Willis |
| Back Country Ranch | farm | Pearisburg |
| BACKCOUNTRY | farm, wilderness lodges | Glade Spring, VA |
| Beagle Ridge Herb Farm | farm, education center | Wytheville |
| Big Stone Gap Farmers Market | farmers market | Big Stone Gap |
| Blacksburg Farmers' Market | farmers market | Blacksburg |
| Blacksnake Meadery | farm winery | Dugspur |
| Bluefield Farmer's Market | farmers market | Bluefield |
| Brooks Mill Winery | winery | Wirtz |
| Bull Pine Hollow Farm | farm | Radford |
| Chateau Morrisette | winery | Floyd |
| Clements Cherry Orchard | farm | Ararat |
| Clinch River Farmer's Market | farmers market | St Paul |
| Coltsfoot Winery | farm winery | Abingdon |
| Davis Bros Nursery | farm | Rose Hill |
| Davis Valley Winery | farm | Rural Retreat |
| Doris's Daylilies | farm, home garden | Wirtz |
| Dreamland Alpacas LLC & Farm Store | farm | Meadowview |
| Flying Flowers | walk through butterfly house | Wytheville |
| Foggy Ridge Cider | winery-hard cider making | Dugspur |
| Foster's Greenhouse & Country Market | farmers market | Rocky Mount |
| Galax Farmers Market | farmers market | Galax |
| Henley Mountain Berry Farm | farm | Wytheville |
| Homestead Creamery Inc | farm | Wirtz |
| Indian Springs Alpacas | farm | Mouth of Wilson |
| Kanawha Valley Arena | farm | Dugspur |
| Kimberly's Greenhouse at Pisgah | retail greenhouse | Tazewell |
| King's Country Produce Inc. | retail grocery, bulk food | Wytheville |
| Laughing Water Farm | farm | Marion |
| Lebanon Farmer's Market | farmers market | Lebanon |
| Lost World Ranch | farm, festival, camel rides llama rides and camel wagon team | Burkes Garden |
| Lyttle Farm of Copper Ridge | farm | Castlewood |
| Maison Beliveau, LLC | farm | Blacksburg |
| Misty Meadows Farm | farm | Penhook |
| Mountain Meadow Farm and Craft Market | farm | Meadows of Dan |
| MountainRose Vineyards, Inc. | vineyard and winery | Wise |
| Mt. Vale Vineyards | vineyard and winery | Galax |
| Nature Way Country Store | bulk food store | Pearisburg |
| Peaceful Heart Alpacas and Farm Store | farm | Fancy Gap |
| Pleasant Hill Farm Field of Flowers | farm, flowers for cutting, weddings, white doves | Max Meadows |
| Punkin Patch Farm | farm | Nickelsville |
| Rixey's Market | country store or general merchandise - retail | Independence, |
| Rural Retreat Winery & Dye's Vineyards | farm | Rural Retreat |
| Sandy Head Ostrich | farm | Tazewell |
| Sinking Creek Farm | farm | Newport |
| Sinkland Farms | farm, festival | Christiansburg |
| Southwest Virginia 4-H Educational Center, Inc. | educational center | Abingdon |
| Southwest Virginia Farmers Market | farmers market | Hillsville |
| Spurlock Gardens | farm | Willis |
| Thee Red Barn | agritourism, corn maize, etc. | Boones Mill |
| Villa Appalaccia Winery | winery | Floyd |
| VILLA APPALACCIA WINERY | farm | Floyd |
| Vincent's Vineyard | farm, farm winery/ sheep farm | Lebanon |
| Wall Farm - Lovely Mount Goat Farm | farm, boer goats | Radford |
| West Wind Farm Vineyard & Winery | farm, vineyard & farm winery | Max Meadows |
| Whispering Winds Daylily & Iris Farm | farm | Moneta |
| Wolf Creek Farm | farm, b&b | Ararat |
| Wytheville Farmers Market | farmers market | Wytheville |
